Turbinicarpus pseudopectinatus grows at a higher altitude than true Chihuahuan Desert in grasslands with virtually no woody vegetation and in pinyon-juniper woodlands. All the sites have gravelly calcareous soils. It also occurs in montane matorral. This species occurs at about ten locations and is not severely fragmented. Illegal collecting is the main threat however the rate of illegal collection is likely to be lower than the natural recruitment rate.
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